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Confocal Laser Scanning Microscopy (CLSM)

(Kopie 2)

In the case of Confocal Laser Scanning Microscopy (CLSM) a laser beam is focussed on the surface of the sample. The reflected light is detected behind a pinhole aperture. This technique enables a lateral resolution that is one third better in comparison to a classic wide field microscope. By focussing the laser, the sample is scanned level by level, thus producing a three-dimensional topographic image. Here the location of maximum reflection along the beam axis is defined as a point on the surface. The intensity image produces a high-contrast microscopic image. At the same time, a classic wide field microscopic image is captured.

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